ORI’s Upgraded "Forensic Droplets" for the quick examination of scientific images
Droplets are small "desktop" applications in Adobe Photoshop®, that automatically process files dragged onto their icon. Droplets are created by recording and saving sequences of steps in the Actions Palette. Each Droplet can be copied to the desktop, to create a near seamless interface for quickly examining the details of a scientific image in Photoshop while reading the publication in the FULL TEXT (html) form or in the PDF form in an Internet Browser.
USE: From within the Internet Browser, drag and drop the fully expanded image over the icon of the saved Droplet, and the latter will open Photoshop and complete a sequence of pre-recorded actions. If Photoshop is already running in the background, 'runtime' will appear almost instantaneous (i.e., in the absence of dialogue and setting options). However, ORI's Prototype Droplets were created for educational purposes, with relevant dialogue and settings features active, to permit user experimentation. After a Droplet has completed its action, the steps can be reviewed by going to the History Palette and "sliding" back and forth through the steps.
The user can create new droplets from the provided Actions.
FORENSIC DROPLETS (CS2 and newer)
Unzip the files below your computer's Desktop and then follow the directions above
DROPLETS FOR COMPARING TWO IMAGES:
The following set of Droplet compare two images, and so they require that Photoshop first be running with the both images already open:
Color Coded Overlay: Any Droplets in this category overlays two images, and color codes each so that individual features in the overlap can be linked back to their respective image.
To use, drag and drop the first image into the Photoshop application. Next, drag and drop the second image onto the icon of the Droplet, and the sequence will proceed.
The specific color scheme may vary, but in general regions where dark features overlap will appear RED, and areas of overlapping background will appear CYAN. Features unique to one or the other image will be WHITE or BLACK. If the same object is common to both images it 1) must be RED and 2) must have a uniform boundary of either BLACK or WHITE. The image can be resized and reoriented using "Free Transform" in the Edit Menu to test alignment of the images. The method of comparison is completely analogous to the widely used colocalization technique that is a widely used and accepted method in cell biology.
The color-coding for features in the overlapped image that are not common to both sources may vary depending on the type of image. In this case, a useful technique for identifying the source of any features is to pre-label each respective image with a large letter or number in the same relative location, such as the upper left corner. The color of the non-overlapping portion of the respective labels will then serve to identify the origin of any specific feature in question.
The specific Droplets for Image Overlay are as follows:
Comments are Welcome:
Simply email ORI using "Forensic Droplets" in the subject line.