Photoshop Videos Illustrating the Guidelines for Appropriately Handling Images.

Please click on each guideline below for video illustration. Also, read the detailed description of all twelve guidelines.

	Treating Images as Data: Digital scientific images should be treated as data			Filters Degrade Data: Use of software filters to improve image quality is usually not recommended for biological images.
	Saving the Original: Manipulations of digital images should always be done on a copy of the raw image data. The original must be retained.			Cloning Degrades Data: Cloning objects into an image or from other parts of the image is very questionable.
	Making Simple Adjustments: Simple adjustments to the entire image are usually acceptable.			Making Intensity Measurements: Intensity measurements of digital images should be performed on raw data and the data should be calibrated to a known standard.
	Cropping is usually OK: Cropping an image is usually acceptable.			Lossy Compression Degrades Data: Avoid the use of lossy compression.
	Comparing Images: Digital images that will be compared to one another should be acquired under identical conditions.			Issues With Magnification: Magnification and resolution issues are important.
	Manipulating the Entire Image: Manipulations that are specific to one area of an image and are not performed on other areas are questionable.			Issues With Pixels: Be careful when changing the size (in pixels) of a digital image.